Physiological and biochemical studies of leaf storage proteins of Glycine max (soybean) by Stephen Francis Klauer

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  • Soybean -- Composition.,
  • Glycoproteins.

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Statementby Stephen Francis Klauer.
The Physical Object
Paginationxii, 157 leaves, bound :
Number of Pages157
ID Numbers
Open LibraryOL16809644M

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Physiological and biochemical studies of leaf storage proteins of Glycine max (soybean). Author(s): Klauer, S. Author Affiliation: Washington State University, Pullman, WAUSA. Journal article: Dissertation Abstracts : S. Klauer. Saline-soluble glycinins and insoluble glutelins are the major storage proteins in soybean (Glycine max) and rice (Oryza sativa), respectively.

In spite of their differences in solubility properties, both proteins are members of the 11S globulin gene family based on their similarities in primary sequences and processing of the coded protein. Wild-type and methionine-modified glycinin.

Soybean (Glycine max (L.) Merrill) is one of the most important legume crops and has a major impact on the global economies [5]. The vigor formation in developing soybean seeds generally starts from physiological maturity (R7) period.

During this period, the developing seeds are susceptible to HTH stress, leading to the reduction of by: 1. Leaf proteins function not only as catalysts but also as major storage sinks of nitrogen.

Some proteins such as ribulose bisphosphate carboxylase (RuBP-Case) act as both a catalyst and a storage protein. The turnover characteristic of individual proteins depends a great deal on their intracellular location and their accessibility to leaf by: THE JOURNAL OF BIOLOGICAL CHEMISTRY 0 by The American Society of Biological Chemists, Inc Vol.No.

20, Issue of J pp. Printed in &. The Glycosylated Seed Storage Proteins of Glycine max and Phaseolus vulgaris STRUCTURAL HOMOLOGIES OF GENES AND PROTEINS* (Received for publication, October 4,). Protein storage vacuoles (PSVs) are specialized vacuoles devoted to the accumulation of large amounts of protein in the storage tissues of plants.

In this study, we investigated the presence of the storage vacuole and protein trafficking to the compartment in cells of tobacco (Nicotiana tabacum), common bean (Phaseolus vulgaris), and Arabidopsis leaf tissue. Soybean (Glycine max L. Merr.) contains two related and abundant proteins, VSPα and VSPβ, that have been called vegetative storage proteins (VSP) based on their pattern of accumulation, degradation, tissue localization, and other characteristics.

To determine whether these proteins play a critical role in sequestering N and other nutrients during early plant development, a VspA antisense. In soybean (Glycine max), increased sink demand due to N 2 fixation, compared with nitrate-fed plants, resulted in higher rates of photosynthesis and delayed leaf senescence (Kaschuk et al., ).

Growth of plants at elevated atmospheric [CO 2 ], which alters source supply, has provided further evidence for the coordination of source. Glycine is the simplest (and the only achiral) proteinogenic amino acid, with a hydrogen atom as its side chain.

It has a role as a nutraceutical, a hepatoprotective agent, an EC (glycine hydroxymethyltransferase) inhibitor, a NMDA receptor agonist, a micronutrient, a fundamental metabolite and a neurotransmitter. It is an alpha-amino acid, a serine family amino acid and a. Growth, physiological and biochemical measurements.

To evaluate the growth status of ica seedlings under drought conditions, growth-related parameters were determined in 24 seedlings from each treatment at the initial stage of water stress and at the final harvesting time, including the leaf number, length and width. After drought stress, the contents of auxin, abscisic acid and.

Abstract. Considerable information is now available concerning the 7 S seed storage proteins of legumes and the genes that encode them.

Our study compares the gene encoding a beta-type subunit of phaseolin (Pvu beta), the 7 S protein of common bean (Phaseolus vulgaris), with the gene encoding an alpha'-subunit of beta-conglycinin (Gma alpha'), the 7 S protein of soybean (Glycine max).

Comparison of NLL conglutins to other legume sequences. Seed storage protein homologues from Glycine max (soybean), Pisum sativum (pea), Arachis hypogaea (peanut), Medicago truncatula and Lupinus albus (white lupin) that had BLAST sequence alignment scores greater than when compared to any of the 16 NLL conglutin protein sequences were identified from the NCBI non-redundant protein.

Soybean [Glycine max (L) Merr.] has been used as a model plant species for studies on plant physiology, biochemistry, and molecular biology. Introduction of foreign genes of interest will benefit these studies and increase soybean values, if transgenic soybean can be developed routinely.

ABA modulates various physiological and biochemical processes by differentially expressing the ABA receptor that is PYR/PYL family gene, SnRK2 (serine/threonine-protein kinase) and ABA-responsive element binding genes and proteins.

Transcription of genes such as 9-cis-epoxycarotenoid dioxygenase (NCED), zeaxanthin epoxidase (ZEP), and ABA 8. PDF | OnRACHANA S. WAKEKAR and others published Tissue specific Cyto-biochemical and Molecular Alterations Associated with Floral Bud Distortion in Soybean (Glycine max. Seeds of many plant species are green during embryogenesis.

To directly assess the influence of light on the physiological status of green oilseeds in planta, Brassica napus and soybean (Glycine max) seeds were rapidly dissected from plants growing in the light or dark.

The activation state of malate dehydrogenase, which reflects reduced thioredoxin and NADP/NADPH ratios, was found to be as. Morphological and Physiological Responses of Seven Different Soybean (Glycine Max L.

Merr.) Cultivars to Drought Stress December Journal of Crop Science and Biotechnology 22(4) In legumes, seed storage proteins are important for the developing seedling and are an important source of protein for humans and animals. Lupinus angustifolius (L.), also known as narrow-leaf lupin (NLL) is a grain legume crop that is gaining recognition as a potential human health food as the grain is high in protein and dietary fibre, gluten-free and low in fat and starch.

There are two confirmed pathways for the uptake and translocation of MONPs in the plant systems: root-to-leaf/fruit or leaf-to-root pathway (Ma et al., a).On one hand, the studies on root-to-leaf/fruit pathway are either through hydroponic (Hernandez-Viezcas et al., ) or soil culture (Rico et al., a), and the reported translocation of MONPs start from seedlings (Servin et al.,   SDS-PAGE patterns of Glycine max seeds proteins.

[ILLUSTRATION OMITTED] The molecular weights of the Glycine max TGXD proteins are shown in Table 1 below. Discussion In this study, the biochemical characterization of soybean (Glycine max) TGXD seed proteins revealed 21 bands representing the proteins present in the soybeans. This study develops differential protein profiles of soybean (Glycine max) seeds (cv.

Saedanbaek and Daewon) varying in protein (% and %) and oil (% and %) contents, using. Emmanuel Oladeji Alamu, Gondwe Therese, Phumzile Mdziniso, Maziya-Dixon Bussie, Assessment of nutritional characteristics of products developed using soybean (Glycine max (L.) Merr.) pipeline and improved varieties, Cogent Food & Agriculture, /, 3, 1, ().

A combined proteomic approach was applied for the separation, identification, and comparison of two major storage proteins, β-conglycinin and glycinin, in wild (Glycine soja) and cultivated (Glycine max) soybean -dimensional polyacrylamide gel electrophoresis (2D-PAGE) with three different immobilized pH gradient (IPG) strips was an effective method to separate a large number of.

These primary studies include numerous soybean growth forms, various stress and experimental treatments, and a range of elevated [CO 2] levels (from to p.p.m.), with a mean of p.p.m. across all studies. Stimulation of soybean leaf CO 2 assimilation rate with growth at elevated [CO 2] was 39%, despite a 40% decrease in stomatal.

Physiological and Biochemical Responses of Vicia Faba Plants to Foliar storage proteins, transcription factors, and replication proteins. Annu Rev Biochem – CAS Article PubMed Google Scholar Effect of zinc and lead toxicity on some physiological parameters of Glycine max L.

J Biomed Environ Sci 4(4)– Soybean (Glycine max (L.) Merr.), which is a rich source of protein and edible oil, has many local names, for example, large bean or yellow bean in China, “ edamame ” in Japan (Shurtleff and Aoyagi, ), and the miracle bean or golden bean in the genus Glycine comprises two subgenera, one being Soja and the other, subgenus Glycine consists of 25 perennial species.

Being an excellent source of protein and calcium (surpassing other major staples such as wheat and sorghum), soybean (Glycine max [L.] Merr) is generally considered the most valuable legume from being produced for human consumption, the plant is used to feed livestock – hence further increasing its significance as a food source [1, 2].

Glycine-rich proteins (AAF and BAD) are the structural proteins of cell walls (Ringli et al. The ubiquitin–protein ligase system (pentameric polyubiquitin, CAA) plays a role not only in protein synthesis, but also in trichome development (Downes et al.

The reflectance spectrum of a leaf and changes in this reflectance spectrum can be a useful source of biochemical and physiological information.

In some cases reflectance‐based measurements have been developed for high‐throughput phenotyping applications (e.g. measurement of the content of leaf chlorophyll and other foliar pigments).

Yuanxin Guo, Runqiang Yang, Hui Chen, Yu Song, Zhenxin Gu, Accumulation of γ-aminobutyric acid in germinated soybean (Glycine max L.) in relation to glutamate decarboxylase and diamine oxidase activity induced by additives under hypoxia, European Food Research and Technology, /sy,4, (), ().

Wild soybean (Glycine soja) is a highly adaptive plant species which can grow well in saline-alkaline soils. In soybean genome, there exist about HD-Zip (Homeodomain-leucine Zipper) genes.

HD-Zip transcription factor family is one of the largest plant specific superfamilies and plays important roles in response to abiotic stresses.

Although HD-Zip transcription factors have been. A combined metabolomic, biochemical, fluxomic, and metabolic modeling approach was developed using 19 genetically distant maize (Zea mays) lines from Europe and America.

Considerable differences were detected between the lines when leaf metabolic profiles and activities of the main enzymes involved in primary metabolism were compared. During grain filling, the leaf metabolic composition.

GMCP3 of Glycine max is another cathepsin F-like CysProt induced by senescence and diverse stresses in nonseed tissues (Esteban-Garcia et al., ). As well as the CysProt from different classes aforementioned, there are other studies regarding the role of CysProt in leaf senescence. Fujiwara K, Motokawa Y.

Mechanism of the glycine cleavage reaction. Steady state kinetic studies of the P-protein-catalyzed reaction. J Biol Chem. Jul 10; (13)– Hiraga K, Kikuchi G.

The mitochondrial glycine cleavage system. Purification and properties of glycine decarboxylase from chicken liver mitochondria. Proteins in the plant apoplast are essential for many physiological processes. We have analysed and compared six different infiltration solutions for proteins contained in the apoplast to recognize the most suitable method for leaves and to establish proteome maps for each extraction.

The efficiency of protocols was evaluated by comparing the protein patterns resolved by 1-DE and 2-DE. Patrick Abbot, in Advances in Insect Physiology, Seasonality in social aphid biology. Photoperiodism is the tracking of day (photophase) or night length (scotophase), coupled with the storage, integration and physiological deployment of these phase ratios in the service of seasonal life history schedules.

As herbivorous insects with complex life cycles in temperate regions, aphids. Coping with salt stress involves complicated mechanisms that include developmental, morphological, physiological and biochemical strategies (Taji et al.

Further, salt stress-regulation genes are expressed, which leads to changes in the protein profile that help plants to adapt to salt accumulation (Parker et al. Due to this substrate‐specificity, VPE enables to function in processing of many vacuolar proteins, including seed storage proteins, proteolytic enzymes and defensive proteins (Fig.

The functional differentiation of VPE during evolution of angiosperms might. Water stress is one of the most important physiological stress factors that adversely affect soybeans in many critical aspects of their growth and metabolism.

Soybean’s growth, development and productivity are severely diminished, when soil or cell water potential becomes inadequate to sustain metabolic functioning. However, little has been done to gather comprehensive information regarding. The remaining nitrogen probably comes from the breakdown of specialized storage proteins in the soybean leaf.

In a more recent study (Ohtake et al. ), non-nodulated soybean (Glycine max (L.) Merr.) plants were cultivated hydroponically under 5 m M NaNO 3 (ample N supply) or m M NaNO 3 (deficient N conditions). The physiological performance of the stressed plants was not significantly altered, suggesting that both cultivars were well adapted to mild cold night stress.

The biochemical response of the plants, regarding antioxidant enzymes, H2O2 and TBARS accumulation, confirmed that .Pea as a model for proteomic studies. Several investigations have been presented on alterations of protein profiles of pea under different physiological conditions (Table (Table1).

1).Proteomic approaches for non-model species, like pea, are currently limited because the identification of peptides critically depends on an available sequence database. Foliar iron (Fe) fertilization of crops may increase Fe concentrations in edible portions of plants and improve yield in soils with low available Fe.

However, the role of foliar Fe fertilization in increasing seed Fe has not been studied in soybeans (Glycine max). In this study, the Pheroid® nutrient delivery technology was combined with FeSO4 or nanostructured FePO4 to develop potential new.

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